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How to Reconstitute Peptides (For Research Use Only) Note:

Note: The information provided below is for educational and research purposes only. Spectrum Peptides products are not intended for human consumption, medical, or veterinary use. Always follow proper laboratory protocols.


🧪 What You’ll Need:

  • Lyophilized peptide (in powder form)
  • Sterile bacteriostatic water or sterile saline (depending on research needs)
  • Sterile syringe and needle
  • Alcohol swab
  • Vial adapter (optional)

🧫 Step-by-Step Reconstitution Guide:

  1. Sanitize Your Work Area
    • Wipe down all surfaces and tools with alcohol to maintain sterile conditions.
  2. Choose a Suitable Diluent
    • Bacteriostatic water is most commonly used due to its longer shelf-life after reconstitution.
    • Use sterile saline or acetic acid only if your research protocol specifies it.
  3. Determine Volume
    • Calculate how much diluent you need based on your desired concentration (e.g., 1 mg of peptide + 1 mL diluent = 1 mg/mL concentration).
  4. Draw the Diluent
    • Use a sterile syringe to draw the appropriate amount of diluent.
  5. Inject the Diluent Into the Peptide Vial
    • Slowly inject the diluent into the vial, aiming it against the glass wall to avoid foaming.
    • Do not shake the vial. Gently swirl or tilt it to mix.
  6. Allow Peptide to Fully Dissolve
    • Let the vial sit at room temperature for a few minutes until the peptide is fully dissolved.
    • Swirl again gently if needed.
  7. Label and Store
    • Clearly label the vial with date and concentration.
    • Store in a refrigerator (2°C to 8°C) unless otherwise specified in your lab’s protocol.

⚠️ Important Notes:

  • For laboratory research only. Not for human use.
  • Use reconstituted peptides promptly and discard any unused solution according to lab disposal guidelines.
  • Avoid repeated freeze-thaw cycles.